Glycerol Ester Hydrolase Activity of Propionibacterium shermanii
نویسندگان
چکیده
منابع مشابه
Purification and properties of a glycerol ester hydrolase (lipase) from Propionibacterium shermanii.
An intracellular glycerol ester hydrolase (lipase) from Propionibacterium shermanii was recovered from cell-free extracts and purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography on diethylaminoethylcellulose. Maximum enzyme activity was observed at pH 7.2 and 47 C when an emulsion of tributyrin was used as substrate. The enzyme was stable between pH 5.5 a...
متن کاملPropionic acid production in glycerol/glucose co-fermentation by Propionibacterium freudenreichii subsp. shermanii.
Propionibacterium freudenreichii subsp. shermanii can ferment glucose and glycerol to propionic acid with acetic and succinic acids as two by-products. Propionic acid production from glucose was relatively fast (0.19 g/Lh) but gave low product yield (~0.39 g/g) and selectivity (P/A: ~2.6; P/S: ~4.8). In contrast, glycerol with a more reduced state gave a high propionic acid yield (~0.65 g/g) an...
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A rapid and accurate method is described for the assay of cholesterol ester hydrolase (CEH) activity. Aliquots of the enzyme-substrate incubation mixture are extracted into isopropanol. The free cholesterol concentration in each extract is determined enzymatically using a single aqueous reagent containing cholesterol oxidase and peroxidase. The free cholesterol remaining after the cholesterol e...
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Propionibacteria have been reported as being oxygen sensitive bacteria. Nevertheless, in this work, we intend to show that Propionibacterium shermanii CDB 10014 is able to grow even at high volumetric oxygen transfer coefficients (KLa). Similar final cell concentrations were achieved in experiments at different KLa values, with increasing cell yield from 0.08 g/g cells in anaerobic con ditions...
متن کاملEffect of ultrasonic treatment on metabolic activity of Propionibacterium shermanii, cultivated in nutrient medium based on milk whey
Utilization of milk whey still remains one of the most serious problems of the modern world dairy industry. Because of high biological value of whey it can be used as nutrient media in techniques of direct microbiological synthesis of complex high-molecular substances, for example the B12 vitamin, which participate in various biocatalytic reactions in organism providing normal functioning of th...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1970
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(70)86258-0